Bicine is named 2-(Bis (2-hydroxyethyl) amino) acetic acid) N. The bicine buffer (150 25 4) is an amphoteric amino acid buffer. It has activity in the range of pH 7.6-9.0 (PKA is 8.3 at 25 ° C). Due to the bicine structure, it is the recommended buffer for low-temperature biochemical work.

Bicine buffer produced by Desheng is widely used. Bicine buffer is not only used to prepare stable substrate solution for guaninase determination, but also used as electrophoresis buffer. Bicine buffer is used as spacer arm in the fractionation of plasma protein by isokinetic electrophoresis with cross-linked dextran. Bicine buffer is also a necessary condition to maintain the constant pH value of the electrophoresis system.

Bicine Buffer (150-25-4) Detailed Introduction:

Bicine buffer is an organic compound used as a buffer. It is one of good's buffers with a pKa of 8.35 at 20 ° C. It is prepared by reacting glycine with ethylene oxide and then hydrolyzing the lactone. Bicine is formed by amine degradation in the presence of O2, H2S or thiosulfate. It is a zwitterionic biological buffer containing amine and acid group.

Bicine Buffer (150-25-4) Advantages:

Bicine buffer can combine with hydroxyl radical and form formaldehyde in the reaction process;

When bicine is used in the presence of an enzyme with oxidase activity, it may form triazine nitrogen oxide free radicals. Compared with other buffers, the yield of glycine isolated small peptides in bicine buffer is higher;

Bicine and luciferase can produce more light in the presence of ATP.

Desheng Advantage On Bicine Product:

Bicine buffer produced by Desheng company has purity ≥ 98% and chloride ion content < 0.01%;

Stable product performance, with R & D and production team, some indicators can be customized according to customer needs;

The price of the source manufacturer is more advantageous, and the quantity is large, and the price is more discounted;

It has rich technical knowledge reserves and can provide efficient technical support;

The maximum daily output can reach 1-2 tons, which can be supplied stably for a long time;

There are more than 100 exporting countries with rich export experience.

Bicine Product Application:

Recommended buffer for low temperature biochemical work;

Electrophoresis buffer;

Buffer for enzyme reaction;

Buffer in concentrated solution of trace elements in sea salt;

Bicine buffer is used as chelating agent in soil remediation;

Effective inhibitors for oxidative degradation of amines;

As a buffer for cell culture research;

he spacer arm in the fractionation of plasma proteins by isokinetic electrophoresis with cross-linked dextran.

Bicine Product Usage Method

Preparation of Bicine solution: for experiments related to cell culture, dissolve the configuration according to the configuration method below. If it is used for cell experiment, please prepare it into liquid and filter it with 0.22um before adding cells.

Preservation of storage liquid: it is recommended to prepare and use it now, and the liquid is not very stable; It can also be sub packaged into a single dosage, which is stable for 2 years. Avoid repeated freezing and thawing.

Configuration method of bicine buffer:

(1) Prepare 0.1M solution (a): bicine16 317g / 1000ml deionized water

(2) Prepare 0.1mnaoh solution (b): naoh4g / 1000ml deionized water

(3)pH5. 1 1,000ml(A)+0ml(B) (A):(B)=5:0

pH7. 8 1,000ml(A)+200ml(B) (A):(B)=5:1

pH8. 2 1,000ml(A)+400ml(B) (A):(B)=5:2

pH8. 6 1,000ml(A)+600ml(B) (A):(B)=5:3

pH10. 4 1,000ml(A)+800ml(B) (A):(B)=5:4

Bicine Note:

Bicine buffer products of our company have unique ingredients and processes. Please do not mix bicine products produced by Desheng with other suppliers to avoid product contamination;

When preparing the solution for the experiment, the required container shall be cleaned with pure water for more than 3 times before use to avoid mixing with other substances;