Isoluminol is a chemiluminescence immunoassay substance. The luminescence principle of the isoluminol chemiluminescence is that it is catalyzed by horseradish peroxidase under alkaline conditions and is oxidized by hydrogen peroxide to generate an excited intermediate of 3-aminophthalic acid. ,emits a photon when it returns to its ground state.

Isoluminol, also known as 4-aminophthalic hydrazide, its luminescence is oxidation reaction luminescence. 4-aminophthalhydrazide was mainly used for the determination of inorganic and organic biological small molecules in the early days, and now it has also been widely used in the fields of gene and immunoassay etc fields.

Isoluminol in Detail

Although the quantum yield of isoluminol is only 1/10 of that of luminol, after its aromatic amino group is reacted, the quantum yield can be enhanced by about 10 times. Therefore, when these substances are directly used as labels, isoluminol isoluminol chemiluminescence is often used. or its derivatives. Isoluminol and its derivatives are used as chemiluminescent labels for a range of steroids, hormones and other substances.