Principle Of Rotavirus Diagnostic Test, Rotavirus Antigen Test

Rota Test Strip is a qualitative immunochromatographic assay for the determination of Rotavirus in stool samples. The membrane is pre‐coated with mouse monoclonal antibodies, on the test band region, against viral antigens. During testing, the sample is allowed to react with the colored conjugate (anti‐Rotavirus mouse monoclonal antibodies‐red microspheres) which was pre‐dried on the test. The mixture then moves upward on the membrane by capillary action. As the sample flows through the test membrane, the colored particles migrate. In the case of a positive result the specific antibodies present on the membrane will capture the colored conjugate. The mixture continues to move across the membrane to the immobilized antibody placed in the control band region, a GREEN colored band always appears.