E07G0462 Porcine Glucose ELISA kit

The Porcine Glucose ELISA kit can be used to identify samples from the porcine species. Glucose can also be called GBA, GC, GLUC, GBA1, Beta-GC, Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, Beta-glucosylceramidase 1.

Principle of the Assay

GLUC ELISA kit uses an anti-GLUC antibody and an GLUC-HRP conjugate in a competitive enzyme immunoassay method. GLUC-HRP conjugate is incubated with the assay sample and buffer in a pre-coated plate for one hour. The wells are decanted and washed five times when the incubation period is over. The HRP enzyme substrate is then incubated in the wells. The result of the enzyme-substrate reaction is a complex that is blue in hue. The process is finally stopped by adding a stop solution, causing the solution to turn yellow. In a microplate reader, the color intensity is measured spectrophotometrically at 450 nm. Due to competition for the anti-GLUC antibody binding site between GLUC from samples and GLUC-HRP conjugate, the intensity of the color is inversely proportional to the concentration of GLUC. Since the number of sites is limited, as more sites are occupied by GLUC from the sample, fewer sites are left to bind GLUC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The GLUC concentration in each sample is interpolated from this standard curve.

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