Principle of the Assay

Active caspase 3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-Active caspase 3 antibody and an Active caspase 3-HRP conjugate. The assay sample and buffer are incubated together with Active caspase 3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Active casp3 elisa concentration since Active caspase 3 from samples and Active caspase 3-HRP conjugate compete for the anti-Active caspase 3 antibody binding site. Since the number of sites is limited, as more sites are occupied by Active caspase 3 from the sample, fewer sites are left to bind Active caspase 3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Active caspase 3 elisa concentration in each sample is interpolated from this standard curve.

Citations of Human Active Caspase 3 ELISA kit

E01A0015 has been referenced in the below publications:

Caspases as prognostic markers and mortality predictors in acute organophosphorus poisoning.

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